Multiple Reaction Monitoring to Identify Site-Specific Troponin I Phosphorylated Residues in the Failing Human Heart Running title: Zhang et al., Targeted quantitation of cardiac troponin I phosphorylation
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Multiple reaction monitoring to identify site-specific troponin I phosphorylated residues in the failing human heart.
BACKGROUND Human cardiac troponin I is known to be phosphorylated at multiple amino acid residues by several kinases. Advances in mass spectrometry allow sensitive detection of known and novel phosphorylation sites and measurement of the level of phosphorylation simultaneously at each site in myocardial samples. METHODS AND RESULTS On the basis of in silico prediction and liquid chromatograph...
متن کاملHeart Failure Multiple Reaction Monitoring to Identify Site-Specific Troponin I Phosphorylated Residues in the Failing Human Heart
Background—Human cardiac troponin I is known to be phosphorylated at multiple amino acid residues by several kinases. Advances in mass spectrometry allow sensitive detection of known and novel phosphorylation sites and measurement of the level of phosphorylation simultaneously at each site in myocardial samples. Methods and Results—On the basis of in silico prediction and liquid chromatography/...
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Posttranslational modifications (PTMs) are chemical or physical changes to proteins that can alter structure/ function relationships and thus influence catalysis, cell localization, and interactions with other proteins and biomolecules. There are 200 recognized PTMs, generating a vast scope for altering protein function and increasing the potential for complex regulatory crosstalk between diver...
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Introduction: Coping ways with stress in coronary heart disease patients can lead to significant changes in levels of Cardiac Biomarker Troponin I, but researchers had not explored it empirically. So, the main objective of this study was to identify the relationship between coping ways with stress and Cardiac Biomarker Troponin I and also, finding coping ways that predict changes in the concent...
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s Zhang P, Kooij V, Murphy AM, van der Velden J, dos Remedios C, van Eyk JE (2010). Site‐specific quantification of phosphorylated cardiac troponin I by MS‐based multiple reaction monitoring. International symposium on elctro‐ and liquid‐ phase seperation techniques, Baltimore, USA. Kooij V, Piersma SR, Tang KW, Jiménez CR, Zang P, van Eyk JE, Murphy AM, van der Velden J, Stienen GJM (2010)...
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